Inventors:
Melanie Feola - Cherry Hill NJ, US
Martin Adelson - East Windsor NJ, US
Eli Mordechai - Robbinsville NJ, US
Lisa Novak - Miami FL, US
Assignee:
Medical Diagnostics Laboratories, LLC - Hamilton NJ
International Classification:
C12Q 1/68, C12P 19/34, C07H 21/02, C07H 21/04
US Classification:
435 615, 435 61, 435 611, 435 612, 435 912, 536 231, 536 237, 536 2432, 536 2433
Abstract:
Disclosed are oligonucleotides useful in methods for determining whether a sample contains , a spirochete which is a causative agent of Lyme disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the p24 gene for the outer surface protein of , are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains. Real-time PCR and detection using florescence resonance energy transfer is disclosed.