Inventors:
Leslie Johnston-Dow - Palo Alto CA, US
Lisa Demeter - Rochester NY, US
Camille White - San Diego CA, US
Keming Song - Chesterfield MO, US
Robert Kohlenberger - Burlingame CA, US
Morgan Conrad - Montara CA, US
Angela Myers - Alameda CA, US
International Classification:
C12Q001/70, C12Q001/68, C12P019/34
US Classification:
435005000, 435006000, 435091200
Abstract:
Methods are provided for amplifying regions of the HIV pol gene amplifying double-stranded nucleic acid template derived from HIV tube RT-PCR with novel PCR primers to produce amplified target sequences. Methods are also provided for analyzing the nucleotide sequence of these amplified targets using novel sequencing primers and the data is analyzed. The determined nucleotide sequence can be compared to the sequence of known drug resistance mutations in the HIV pot gene to determine the viral genotype.