Work:
Solazyme, Inc.
Senior Research Associate
The J. David Gladstone Institutes, Gladstone Institute of Neurological Disease - San Francisco, CA
Senior Research Associate
The J. David Gladstone Institutes, Gladstone Institute of Neurological Disease - San Francisco, CA
Research Associate III
The J. David Gladstone Institutes, Gladstone Institute of Neurological Disease - San Francisco, CA
Research Associate II
Iowa State University - Ames, IA
Research/Teaching Assistant
Taiwan Society of Laboratory Medicine Taipei, Taiwan
Research Associate
School of Medicine National Yang-Ming University, Taipei, Taiwan
Research Assistant
National Taiwan University Hospital
Clinical Laboratory Intern
Education:
Iowa State University - Ames, IA
M.S. in Genetics
National Taiwan University, College of Medicine
B.S. in Clinical Laboratory Science and Medical Technology
Skills:
Small Rodent Handling Standard husbandry : Setup crosses, weaning, tagging Behavioral assays: Rotarod, Open field, Elevated plus maze, Balance beam, clasping scoring Dosing: oral gavage, I.P injection, compound treatment Sample collection: perfusion, blood and tissue collection Sample preparation: PFA fixation, brain slices, immunostaining Molecular Biology Cloning: Primer design, Sequence analysis Classical gene cloning (restriction, Gateway technology) Mutagenesis (Quick Change technology) PCR: classical PCR, RT-qPCR, genotyping and electrophoresis DNA, RNA preparations Southern, Northern blot Cellular Biology Cell culture: Cell lines (HEK293, COS, HepG2, BV2, Raw) Generation of stable cell lines Transfection: Electroporation, CaPO4, Lipofectamine Microscopy: Immunofluorescence and confocal microscopy Biochemistry Western blot (detection with ECL or Li-Cor) ELISA and other Colorimatric Assays (NAD+/NADH) Protein assays in vitro Filter Trap Cellular Assays Immunoprecipitation Reporter gene assays (luciferase reporter, UPS) Immunostaining Flowcytometry Bioinformatic Vector NTI, Serial Cloner, Graphpad Prism (statistics)