LIDICE LOPEZ, PA
Physician Assistant at 12 Ave, Miami, FL

7625712, Dec 1, 2009

May 17, 2005

11/130492

Ronald D. Paul - Fort Lauderdale FL, US
James L. Wyatt - San Diego CA, US
Barbara Carrillo - Miami FL, US
Oilda Rubio - Miami FL, US
Diana B. Careaga - Miami FL, US
Lidice L. Lopez - Miami FL, US

Beckman Coulter, Inc. - Fullerton CA

G01N 33/567

435 721, 435 723, 435 724, 4352852, 4352871, 4352872, 4352887, 436 10, 436 17, 436 56, 436164, 436171, 436175, 436546, 422 8201, 422 8205, 422 8207

A method useful for the enumeration of cell populations in a biological sample includes the steps of reacting in a single reaction mixture a sample, a first antibody labeled with a fluorochrome having a first emission spectrum and an additional antibody. The first antibody binds to an antigenic determinant differentially expressed on leukocytes and non-leukocytes. The additional antibody binds to an antigenic determinant differentially expressed on mature and immature granulocytes or myeloid cells, and is labeled either with the first fluorochrome or an additional fluorochrome having an emission spectrum distinguishable from the first emission spectrum. The reaction mixture can be mixed with a nucleic acid dye having an emission spectrum that overlaps with one of the first or additional emission spectra. The reaction mixture may be treated with a lytic system that differentially lyses non-nucleated red blood cells and conserves leukocytes. Populations of hematological cells are detected and enumerated using at least two parameters (fluorescence, optical, and electrical) for each population.

2004018, Sep 23, 2004

Mar 20, 2003

10/392518

John Maples - Miami FL, US
Lidice Lopez - Miami FL, US
Nancy Torke - Miami FL, US

Coulter International Corp. - Miami FL

C12Q001/68, C07H021/04

435/006000, 536/025320

A composition for enhancing differential staining of RNA, DNA and granules in a sample comprising cells contains a first fluorescent dye that can bind specific binding sites and non-specific binding sites in the sample. This first dye emits fluorescence at a first wavelength. The composition contains at least an additional component, which is a second non-intercalating dye in the composition that competes with said first dye for binding to the nonspecific binding sites, or a permeabilizing agent to enhance permabilization of the dyes into the cells, or both. The molar ratio of the second dye and the first dye is at least about 20:1.

7674598, Mar 9, 2010

Jun 27, 2006

11/475313

Ronald D. Paul - Fort Lauderdale FL, US
Oilda Rubio - Miami FL, US
Diana B. Careaga - Miami FL, US
Lidice L. Lopez - Miami FL, US
Ravindra Mylvaganam - Longmeadow MA, US

Beckman Coulter, Inc. - Fullerton CA

G01N 33/567

435 724, 435 71, 435 721, 435 723, 4352852, 4352871, 4352872, 4352887, 436 10, 436 17, 436 56, 436164, 436171, 436175, 436546, 422 8201, 422 8205, 422 8207

Methods for differentially identifying cells in an instrument employ compositions containing a combination of selected antibodies and fluorescent dyes having different cellular distribution patterns and specificities, as well as antibodies and fluorescent dyes characterized by overlapping emission spectra which form non-compensatable spectral patterns. When utilizing the compositions described herein consisting of fluorescent dyes and fluorochrome labeled antibodies with overlapping spectra that cannot be separated or distinguished based upon optical or electronic compensation means, a new fluorescent footprint is established. This new fluorescent footprint is a result of the overlapping spectra and the combined cellular staining patterns of the dyes and fluorochrome labeled antibodies chosen for the composition. The new fluorescent footprint results in histogram patterns that are useful for the identification of additional cell populations or subtypes in hematological disease.

7344890, Mar 18, 2008

Nov 9, 2005

11/270859

Carlos A. Perez - Miami FL, US
Lidice L. Lopez - Miami FL, US
Mark A. Wells - Davie FL, US
Joaquin Ibanez - Cooper City FL, US
Eileen Landrum - Miami FL, US
Roberto Del Valle - Coral Gables FL, US
Santiago Galvez - Miami FL, US

Beckman Coulter, Inc. - Fullerton CA

G01N 33/48

436 63, 436 8, 436 10, 436 17, 436164, 436174, 436175, 422 73, 422 8205, 422 8209, 435 2

A method for discriminating and quantifying platelets within an analyzed blood sample involves initially diluting the blood sample with a ghosting reagent that causes a change in the index of refraction of the red blood cells. Owing to the change in the index of refraction, light scattered from the ghosted red blood cells will be substantially reduced relative to light scattered from platelets. This results in locations of platelets within a scatterplot of the analyzed blood sample to fall within a region distinguishable from those containing normal red blood cells, fragmented red blood cells, and microcytic red blood cells.

7208319, Apr 24, 2007

Jan 24, 2006

11/338229

Lidice L. Lopez - Miami FL, US
Carlos A. Perez - Miami FL, US
Mark A. Wells - Davie FL, US
Diana B. Careaga - Miami FL, US
Ziling Huo - Miami FL, US
Cheng Qian - Miami FL, US
Nancy J. Torke - Miami FL, US

Beckman Coulter, Inc. - Fullerton CA

G01N 31/00, G01N 33/48

436 10, 436 63, 436164, 436175, 435 2

Methods are provided for measurement of nucleated red blood cells in a blood sample. The methods include exposing a blood cell sample to a reagent system to lyse mature red blood cells, subsequently analyzing nucleated red blood cells in a flow cell by axial light loss, DC impedance, and medium angle light scatter measurements; by axial light loss, low angle light scatter, and medium angle light scatter measurements; or by axial light loss, DC impedance, low angle light scatter, and medium angle light scatter measurements; and then differentiating nucleated red blood cells from other cell types by using measured signals and/or functions thereof.

7008792, Mar 7, 2006

Feb 1, 2005

11/048086

Lidice L. Lopez - Miami FL, US
Carlos A. Perez - Miami FL, US
Mark A. Wells - Davie FL, US
Diana B. Careaga - Miami FL, US
Ziling Huo - Miami FL, US
Cheng Qian - Miami FL, US

Beckman Coulter, Inc. - Fullerton CA

G01N 31/00, G01N 33/48

436 10, 436 63, 436164, 436175, 435 2

Methods are provided for measurement of nucleated red blood cells. The methods include exposing a blood cell sample to a reagent system to lyse mature red blood cells, subsequently analyzing nucleated red blood cells in a flow cell by axial light loss, low angle light scatter and DC impedance measurements, or two selected measurements thereof; and then differentiating nucleated red blood cells from other cell types by using measured signals and/or functions thereof.