Inventors:
Peter G. Schultz - La Jolla CA, US
Lei Wang - San Diego CA, US
John Christopher Anderson - San Diego CA, US
Jason W. Chin - San Diego CA, US
David R. Liu - Lexington MA, US
Thomas J. Magliery - North Haven CT, US
Eric L. Meggers - Philadelphia PA, US
Ryan Aaron Mehl - San Diego CA, US
Miro Pastrnak - San Diego CA, US
Stephen William Santoro - San Diego CA, US
Zhiwen Zhang - San Diego CA, US
Assignee:
The Scripps Research Institute - La Jolla CA
The Regents of the University of California - Oakland CA
International Classification:
C12N 9/10, C12N 15/12, C12P 21/00
US Classification:
435 6, 435 691, 43525233, 435325, 435193
Abstract:
This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.