LAURENCE COOPER, M.D.
Medical Practice at Holcombe Blvd, Houston, TX

2007003, Feb 15, 2007

Aug 8, 2006

11/500490

Laurence Cooper - Houston TX, US
John Rossi - Alta Loma CA, US

City of Hope - Duarte CA

A61K 48/00, C12N 5/08

424093210, 435372000

The present invention is directed to universal T cells and their use in treating diseases and other physiological conditions. More specifically, the present invention is directed to universal T cells and their use in treating treating B-lineage acute lymphoblastic leukemia (B-ALL) in particular and malignancy in general. The universal T cells contain (i) nucleic acid encoding a chimeric antigen receptor (CAR) to redirect their antigen specificity and effector function and (ii) nucleic acids encoding shRNA and/or siRNA molecules to down-regulate cell-surface expression of T cell classical HLA class I and/or II genes to avoid recognition by recipient T cells. The universal T cells may also contain a nucleic acid encoding a non-classical HLA gene, such as an HLA E gene to enforce expression of HLA E genes and/or an HLA G gene to enforce expression of HLA G genes, to avoid recognition by recipient NK cells. The universal T cells may further contain a nucleic acid encoding a selection-suicide gene.

2011010, May 5, 2011

Oct 30, 2009

12/610133

Laurence J.N. Cooper - Houston TX, US
Dean A. Lee - Pearland TX, US
Lisa Biswal - Houston TX, US
Robert Raphael - Houston TX, US
Thomas C. Killian - Houston TX, US

Office of the Board of Regents - Austin TX

A61K 45/00, C12M 3/00, A61P 25/28, A61P 7/06

424 9321, 4352852

This invention generally relates to devices and methods for transfection of living cells using electroporation, in particular high throughput microfluidic electroporation, and to therapeutic uses of the transfected cells.