MR. LARRY WAYNE GREEN, RPH, CDE
Pharmacy in Tacoma, WA

6767733, Jul 27, 2004

Oct 10, 2001

09/974089

Larry R. Green - Tacoma WA

PriTest, Inc. - Redmond WA

C12M 134

4352885, 435 4, 4352872, 4352887, 422 8211, 436164

The present invention concerns a portable, reliable and sensitive biosensor apparatus. In certain embodiments, the apparatus incorporates a fluidic cube, comprising a vent cap, vent cap isolator, cube body, waveguide and stage. In preferred embodiments, the fluidic cube further comprises a fluid manifold. In more preferred embodiments, the fluidic cube comprises one or more sample channels that are designed to increase fluid mixing through the use of baffles. The biosensor is designed to simultaneously process multiple samples for a variety of analytes. In certain embodiments, the biosensor is designed to operate as a compact, stand-alone automated unit that can reliably analyze environmental, clinical, veterinary, pathologic or medical samples under adverse field conditions.

2004002, Feb 5, 2004

Dec 28, 2001

10/035367

Larry Green - Tacoma WA, US

G06K019/06

235/491000

The present invention concerns methods of luminescent identification and calibration. In certain embodiments, items are simultaneously attached to a luminescent label, one or more luminescent calibration spots and one or more sample spots containing binding moieties. In preferred embodiments, the binding moieties are antibodies. In other preferred embodiments, target molecules that bind to the binding moieties are detected using the same luminescent tag as is found in the luminescent label and the calibration spots. In more preferred embodiments, the luminescent tag is Alexa Fluor 647. The label is of use to identify each array and the binding moieties found in each sample spot. The calibration spots are of use to more accurately quantify the amount of target compound attached to each sample spot. The methods provide for a more rapid preparation of labeled arrays of binding moieties, while minimizing the possibility of array contamination.

2004001, Jan 29, 2004

Apr 22, 2002

10/128608

Larry Green - Tacoma WA, US

G01N033/53

435/007100

The present invention discloses compositions and methods for the detection of infective agents (prions) associated with transmissible spongiform encephalopathies. More particularly, the present invention involves compositions and methods for detection and diagnosis of “mad cow” disease and vCJD. In certain embodiments, prions are treated to remove bound lipids before immunodetection. In other embodiments, hydrophobic probes are used to collect prions from oral or anal tissue. Preferred embodiments of the invention involve the use of arrays of binding moieties, such as antibodies, with varying degrees of affinity and specificity for the infective agent. The presence of prions in biological samples may be determined by the pattern of binding of infective agent to the array. The prions may be distinguished from other proteins of similar or identical amino acid sequence, but different secondary, tertiary or quaternary structure, by the different patterns of binding to the array.

2004006, Apr 1, 2004

Apr 29, 2003

10/425222

Larry Green - Tacoma WA, US

G06K019/06

235/491000, 235/494000

The present invention concerns methods and apparatus for detecting and/or identifying analytes, using arrays of binding moieties. In preferred embodiments, the arrays are attached to glass slides. Fluorescent signals obtained from the slides are analyzed by a digital image subtraction method. In preferred embodiments, the glass slides are labeled using a binary code that may be used to identify the lot number and/or date of manufacture of the arrays.