LANCE B KOUTNY
Broker in Methuen, MA

6375817, Apr 23, 2002

Apr 16, 1999

09/293313

Todd A. Taylor - Framingham MA
William W. Carson - Hopkinton MA
Lance Koutny - Methuen MA

PerSeptive Biosystems, Inc. - Framingham MA

G01N 2226

204453, 204604, 422100, 436180

The present invention is directed to apparatus and methods for rapid, automated, microscale sample analysis using pressure differentials. The invention includes an apparatus having intersecting channels for introduction of a sample and separation of that sample into its components. The sample introduction and separation channels preferably are etched in a microfabricated device, such as a microchip, to form a junction. Pressure gradients are applied to the channels to form a sample plug in the separation channel. The separation channel may have disposed within it a medium for separation of the components suspected to be contained in the sample. For example, with the proper medium, a voltage gradient may be applied along the separation channel to separate the components of the sample electrophoretically. The apparatus also may include means for detecting the components of the sample subsequent to separation.

6207031, Mar 27, 2001

Sep 14, 1998

9/153215

Aram S. Adourian - Watertown MA
Daniel J. Ehrlich - Lexington MA
Lance B. Koutny - Methuen MA
Paul T. Matsudaira - Wayland MA
Dieter R. Schmalzing - Cambridge MA

Whitehead Institute for Biomedical Research - Cambridge MA

G01N27/26;27/447

204451

A technique processes a sample of biomolecular analyte. The technique uses an apparatus having a support assembly that receives and supports a test module, a load assembly that loads the sample of biomolecular analyte onto the test module, an electrophoresis assembly that applies a current to the test module such that components within the sample separate by electrophoresis, and a controller that controls operations of the load assembly and the electrophoresis assembly. The load assembly and the electrophoresis assembly are coupled to the support assembly. The controller controls the operation of the load assembly in an automated manner. Preferably, the test module includes a dielectric plate member having an upper planar surface and a lower planar surface that is spaced apart from and coplanar with the upper planar surface. The dielectric plate member has at least one set of channels that includes an injection channel and a separation channel. The injection channel extends from the upper planar surface to the lower planar surface.

6991713, Jan 31, 2006

Jan 23, 2001

09/768075

Aram S. Adourian - Watertown MA, US
Daniel J. Ehrlich - Lexington MA, US
Lance B. Koutny - Methuen MA, US
Paul T. Matsudaira - Wayland MA, US
Dieter R. Schmalzing - Cambridge MA, US

Whitehead Institute for Biomedical Research - Cambridge MA

G01N 27/26, G01N 27/447

204453, 204451, 204455, 204601, 204604, 204605, 422100

A technique processes a sample of biomolecular analyte. The technique uses an apparatus having a support assembly that receives and supports a test module, a load assembly that loads the sample of biomolecular analyte onto the test module, an electrophoresis assembly that applies a current to the test module such that components within the sample separate by electrophoresis, and a controller that controls operations of the load assembly and the electrophoresis assembly. The load assembly and the electrophoresis assembly are coupled to the support assembly. The controller controls the operation of the load assembly in an automated manner. Preferably, the test module includes a dielectric plate member having an upper planar surface and a lower planar surface that is spaced apart from and coplanar with the upper planar surface. The dielectric plate member has at least one set of channels that includes an injection channel and a separation channel. The injection channel extends from the upper planar surface to the lower planar surface.

2006014, Jul 6, 2006

Dec 13, 2005

11/300215

Aram Adourian - Watertown MA, US
Daniel Ehrlich - Lexington MA, US
Lance Koutny - Methuen MA, US
Paul Matsudaira - Wayland MA, US
Dieter Schmalzing - Cambridge MA, US

C07K 1/26

204451000

A technique processes a sample of biomolecular analyte. The technique uses an apparatus having a support assembly that receives and supports a test module, a load assembly that loads the sample of biomolecular analyte onto the test module, an electrophoresis assembly that applies a current to the test module such that components within the sample separate by electrophoresis, and a controller that controls operations of the load assembly and the electrophoresis assembly. The load assembly and the electrophoresis assembly are coupled to the support assembly. The controller controls the operation of the load assembly in an automated manner. Preferably, the test module includes a dielectric plate member having an upper planar surface and a lower planar surface that is spaced apart from and coplanar with the upper planar surface. The dielectric plate member has at least one set of channels that includes an injection channel and a separation channel. The injection channel extends from the upper planar surface to the lower planar surface. The separation channel extends within the dielectric plate member in a plane parallel with the upper and lower planar surfaces and intersects the injection channel.