Inventors:
Kimberly Ann Mislick - Los Angeles CA
Assignee:
California Institute of Technology - Pasadena CA
International Classification:
A61K 3800, A61K 4800, C12N 500, C12N 1500
Abstract:
Methods for controlling transfection efficiency mediated by complexes of cationic species and genetic material by adjusting the amount of membrane-associated proteoglycans and optionally adjusting the plasma concentration of glycosaminoglycans. Transfection efficiency is controlled by the amount of membrane-associated proteoglycans in the cell to be transfected and also by the plasma concentration of glycosaminoglycans. By increasing the amount of membrane-associated proteoglycans in the cell, and optionally decreasing the plasma concentration of glycosaminoglycans, the transfection efficiency can be increased. By decreasing the amount of membrane-associated proteoglycans in the cell, and optionally decreasing the plasma concentration of glycosaminoglycans, the transfection efficiency can be decreased. Transfection efficiency can be controlled, whether performed in vivo, ex vivo, or in vitro.