KIMBERLY D REESE
Cosmetology in Pittsburgh, PA

Janssen R&D - Spring House, PA
Scientist Garnet BioTherapeutics - Malvern, PA
Scientist GlaxoSmithKline, Cardiovascular Discovery Technology Group - Upper Merion, PA, US
Principal Scientist University of Pennsylvania, Department of Cell and Developmental Biology
Postdoctoral Fellow Johns Hopkins University School of Medicine, Department of Molecular Biology and Genetics
Graduate Student Johns Hopkins University School of Medicine
Research Coordinator

Johns Hopkins University School of Medicine - Baltimore, MD
Ph.D. in Biochemistry, Cell and Molecular Biology University of Pennsylvania - Philadelphia, PA
B.A. in Biology

Molecular Biology: PCR, RT-PCR, and qPCR techniques, real-time and Taqman PCR for quantitation and SNP detection, SNP discovery and assay development RNAi using siRNAs, dsRNA, retroviral vectors, and transgenesis in mammalian embryos and cells General molecular biology skills include sub-cloning for fusion protein expression and detailed molecular analysis, DNA, RNA, and protein expression and purification, antibody purification and development, GST-pulldown assays, immunoprecipitation, chromatin immunoprecipitation, cell fractionation, RNA-protein binding assays, yeast-two hybrid screening, Southern, Western (ECL) and Northern blotting, Elisa, MSD assays, and bisulfite mutagenesis. Cell culture and related techniques: broad cell culture experience, especially with embryonic stem cell line creation, derivation and maintenance; Microscopy: Experience with both wide-field and confocal fluorescence microscopy with accompanying immunocytochemistry, histology, and GFP-labeling techniques. Model systems: Extensive work with mice including microinjection, RNAi in mouse embryos, embryo manipulation and culture, dissection and analysis of transgenic mice (standard and RNAi transgenes). Additional experience with human tissues and primary cells, C. elegans, and yeast.