Kent Douglas Evans
Accountancy at Weatherstone Ct, Littleton, CO

2003016, Aug 28, 2003

Apr 27, 2002

10/134655

Min Zhang - Lakewood CO, US
Yat-Chen Chou - Golden CO, US
William Howe - Golden CO, US
Christine Eddy - Golden CO, US
Kent Evans - Littleton CO, US
Ali Mohagheghi - Northglenn CO, US

C12P007/06, C12N001/18, C12N015/74

435/161000, 435/483000, 435/254200

Disclosed in the present invention is a Zymomonas integrant and derivatives of these integrants that posses the ability to ferment pentose into ethanol. The genetic sequences encoding for the pentose-fermenting enzymes are integrated into the Zymomonas in a two-integration event of homologous recombination and transposition. Each operon includes more than one pentose-reducing enzyme encoding sequence. The integrant in some embodiments includes enzyme sequences encoding xylose isomerase, xylulokinase, transketolase and transketolase. The Zymomonas integrants are highly stable, and retain activity for producing the pentose-fermenting enzyme for between 80 to 160 generations. The integrants are also resistant to acetate inhibition, as the integrants demonstrate efficient ethanol production even in the presence of 8 up to 16 grams acetate per liter media. These stably integrated sequences provide a unique Zymomonas that may then be used for the efficient conversion of pentose sugars (xylose, arabinose) to ethanol. Method of using the Zymomonas integrants and derivatives thereof in production of ethanol from cellulosic feedstock is also disclosed. The invention also provides a method for preparing a Zymomonas integrant as part of the present invention. The host Zymomonas strain found particularly useful in the creation of these compositions and methods is Zymomonas mobilis 31821.

8283505, Oct 9, 2012

Jan 5, 2012

13/344460

William A. Evanko - Golden CO, US
Aharon M. Eyal - Jerusalem, IL
David A. Glassner - Littleton CO, US
Fudu Miao - Chester Springs PA, US
Aristos A. Aristidou - Highlands Ranch CO, US
Kent Evans - Littleton CO, US
Patrick R. Gruber - Longmont CO, US
Andrew C. Hawkins - Parker CO, US

Gevo, Inc. - Englewood CO

C07C 29/76

568916

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

8614077, Dec 24, 2013

Nov 22, 2011

13/302775

William A. Evanko - Golden CO, US
Aharon M. Eyal - Jerusalem, IL
David A. Glassner - Littleton CO, US
Fudu Miao - Chester Springs PA, US
Aristos A. Aristidou - Highlands Ranch CO, US
Kent Evans - Littleton CO, US
Patrick R. Gruber - Longmont CO, US
Andrew C. Hawkins - Parker CO, US
Peter Meinhold - Englewood CO, US
Reid M. Renny Feldman - Highlands Ranch CO, US
Uvini Gunawardena - Irvine CA, US
Jun Urano - Aurora CO, US

Gevo, Inc. - Englewood CO

C12P 7/16, C12N 9/10, C12P 17/10

435160, 4352523, 4352542, 435161, 435 2

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

2011012, May 26, 2011

Jun 25, 2010

12/824050

William A. Evanko - Golden CO, US
Mark Brothers - Longmont CO, US
Ken Drobish - Castle Rock CO, US
Aristos A. Aristidou - Highlands Ranch CO, US
Kent Evans - Littleton CO, US
Andrew C. Hawkins - Parker CO, US
Scott Lucas - Parker CO, US

C12P 7/04, C07C 29/74, C12C 11/00, C12M 1/00, C12N 1/00

435157, 568913, 426 11, 4352891, 435243

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allow recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

8101808, Jan 24, 2012

Dec 23, 2008

12/342992

William A. Evanko - Golden CO, US
Aharon M. Eyal - Jerusalem, IL
David A. Glassner - Littleton CO, US
Fudu Miao - Chester Springs PA, US
Aristos A. Aristidou - Highlands Ranch CO, US
Kent Evans - Littleton CO, US
Patrick R. Gruber - Longmont CO, US
Andrew C. Hawkins - Parker CO, US

GEVO, Inc. - Englewood CO

C07C 29/76

568916

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

2013012, May 16, 2013

Aug 15, 2012

13/586599

William A. Evanko - Golden CO, US
Mark Brothers - Longmont CO, US
Ken Drobish - Castle Rock CO, US
Aristos A. Aristidou - Highlands Ranch CO, US
Kent Evans - Littleton CO, US
Andrew C. Hawkins - Parker CO, US
Scott Lucas - Parker CO, US

Gevo, Inc. - Englewood CO

B01D 5/00

435160, 2021852

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allow recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

2013005, Mar 7, 2013

Nov 2, 2012

13/667721

Aharon M. Eyal - Jerusalem, IL
David A. Glassner - Littleton CO, US
Fudu Miao - Chester Springs PA, US
Aristos A. Aristidou - Highlands Ranch CO, US
Kent Evans - Littleton CO, US
Patrick R. Gruber - Longmont CO, US
Andrew C. Hawkins - Parker CO, US

GEVO, INC. - Englewood CO

C12M 1/107, B23P 11/00

4352941, 29428

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

8304588, Nov 6, 2012

Jan 5, 2012

13/344464

William A. Evanko - Golden CO, US
Aharon M. Eyal - Jerusalem, IL
David A. Glassner - Littleton CO, US
Fudu Miao - Chester Springs PA, US
Aristos A. Aristidou - Highlands Ranch CO, US
Kent Evans - Littleton CO, US
Patrick R. Gruber - Longmont CO, US
Andrew C. Hawkins - Parker CO, US

Gevo, Inc. - Englewood CO

C07C 29/76

568916

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.