KEN NICHOLS, P.A
Physician Assistant at Mulberry St, Angleton, TX

License number

Texas PA01042

Professional information

Ken Nichols, Angleton TX - PA (Physician assistant; also Posterior–anterior)

Specialties:

Physician Assistant (PA)

Address:

2327 E Mulberry St, Angleton 77515
(979) 848-8070 (Phone)

Languages:

English

Ken Nichols, Houston TX

Specialties:

Physician Assistant

Address:

5749 San Felipe St, Houston, TX 77057

Apparatus For Cryopreparation, Dry Stabilization And Rehydration Of Biological Suspensions

US Patent:

5780295, Jul 14, 1998

Filed:

Nov 14, 1996

Appl. No.:

8/752740

Inventors:

Stephen A. Livesey - Victoria, AU
Anthony A. del Campo - Houston TX
Abhijit Nag - Houston TX
Ken B. Nichols - Houston TX
Carmen Piunno - The Woodlands TX
David P. Ross - Houston TX

Assignee:

Life Cell Corporation - The Woodlands TX

International Classification:

C12M 102

US Classification:

4353071

Abstract:

This invention relates to methods, apparatus and solutions for cryopreserving microscopic biological materials for biologically extended periods of time. The method comprises treating a suspension of biological material, in an appropriate buffer, with a cryoprotectant or combination of cryoprotectants which raises the glass transition temperature range of the sample. One or more dry protectants may be added to the cryosolution. The cryosolution is then nebulized and rapidly cooled with novel apparatus, dried by molecular distillation, stored and then rehydrated in a buffer prior to its use. The solutions comprise novel mixtures of cryoprotectants.