Inventors:
Kemp B. Cease - Ann Arbor MI
Cortland J. Lohff - Madison WI
Assignee:
The Regents Of The University Of Michigan - Ann Arbor MI
International Classification:
C12N 1500, C12N 1511
Abstract:
Plasmid vectors are provided which enable for the modification of double-stranded DNA which has blunt or one-base 3' overhanging termini, to include any desired four-base 5' overhang sequence, in the reading frame of choice. These vectors enable the cloning of blunt double-stranded DNA sequences or double-stranded DNA sequences having a one-base 3' overhang into a unique restriction site by blunt-end ligation or by one-base 3' overhang cohesive cloning such as T-cloning. The vectors may be amplified and subsequently excised, thereby producing modified fragments free of vector sequences.