Inventors:
Zhenjun Diwu - Sunnyvale CA, US
Jesse J. Twu - Cupertino CA, US
Guoliang Yi - Sunnyvale CA, US
Luke D. Lavis - Sunnyvale CA, US
Yen-Wen Chen - San Francisco CA, US
Kelly J. Cassutt - Somerset NJ, US
Assignee:
MDS Analytical Technologies (US) Inc. - Sunnyvale CA
International Classification:
A61K 49/00, C12Q 1/44, C07D 311/82, C07D 407/00
US Classification:
424 96, 435 19, 549 13, 549223, 549280
Abstract:
Systems, including compositions and methods, for measuring pH, particularly in cells, organelles, and other samples. The compositions include pH-sensitive fluorescent and fluorogenic 2′,7′-dialkylfluorescein derivatives and associated nonfluorescent precursor compounds. The compositions may permit ratiometric measurement in the excitation spectrum and the emission spectrum. The methods include adding a precursor compound to a sample cell, incubating the sample cell to release the free indicator, illuminating the sample cell, and detecting the fluorescence response of the free indicator.