Kathleen U Murphy
Nursing in Spring Valley, CA
License number
Utah 194719-3102
Issued Date
Sep 12, 1990
Expiration Date
Jan 31, 1995
Category
Nurse
Type
Registered Nurse (Under Interstate Compact)
Address
Address
Spring Valley, CA
Education
WEBER STATE UNIVERSITY
Professional information
Method For Releasing Rna And Dna From Cells
US Patent:
5374522, Dec 20, 1994
Filed:
Jun 21, 1991
Appl. No.:
8/711114
Inventors:
Kathleen A. Murphy - Spring Valley CA
Barry D. Epstein - San Diego CA
Ira G. Rosen - San Diego CA
Elizabeth D. Dean - El Cajon CA
Barry D. Epstein - San Diego CA
Ira G. Rosen - San Diego CA
Elizabeth D. Dean - El Cajon CA
Assignee:
Gen-Probe Incorporated - San Diego CA
International Classification:
C12Q 168, C07H 2102, A47J 1906
US Classification:
435 6
Abstract:
A method is disclosed for disrupting cells, including microorganisms, and facilitating thereby the release of cellular components including RNA and DNA into solution. Solutions or suspensions of cells are placed in a container with minute beads of various composition. The container is then placed in an ultrasound bath or otherwise subjected to sonication until the cells disrupt releasing their cellular components, including RNA and DNA. The released RNA and DNA are then available for hybridization with genetic probes.