Inventors:
Robert Chiu - Tustin CA, US
Jun Song - Los Angeles CA, US
International Classification:
C12P 19/34, C07H 21/02, C12N 15/63, C12N 5/00, C12N 15/87
US Classification:
435 913, 536 231, 4353201, 435325, 435463
Abstract:
The present invention provides nucleic acids that include a nucleotide sequence that encodes an siRNA, which nucleotide sequence is operably linked to a target cell-specific promoter RNA polymerase II promoter. The present invention further provides vectors, including expression vectors, which include a subject nucleic acid; and host cells that harbor a subject nucleic acid or a subject expression vector. The present invention further provides methods of modulating (e.g., reducing) expression of a gene in a target cell-specific manner, the methods generally involving introducing into a cell a subject expression vector.