JUDITH E MILLER
Real Estate Commission in Collegeville, PA
License number
Pennsylvania RS190208L
Category
Real Estate Commission
Type
Real Estate Salesperson-Standard
Address
Address
Collegeville, PA 19473
Professional information
Forward Mutation Assay Based On 5-Fluorouracil Resistance
US Patent:
7923204, Apr 12, 2011
Filed:
Apr 22, 2004
Appl. No.:
10/554433
Inventors:
Warren E. Glaab - Doylestown PA, US
Thomas R. Skopek - Collegeville PA, US
Katerina Vlasakova - Hatfield PA, US
Judith E. Miller - Collegeville PA, US
Thomas R. Skopek - Collegeville PA, US
Katerina Vlasakova - Hatfield PA, US
Judith E. Miller - Collegeville PA, US
Assignee:
Merck Sharp & Dohme Corp. - Rahway NJ
International Classification:
C12Q 1/00, C12Q 1/02, C12Q 1/18
US Classification:
435 4, 435 29, 435 32
Abstract:
Disclosed herein is a novel forward mutation assay based on 5-fluorouracil (5-FU) resistance which utilizes a strain of derived from the Ames strain TA100. More specifically, the invention provides a high throughput alternative to the standard Ames mutation assay for the evaluation of the genotoxicity activity of compounds during an early stage of the drug development process. The invention also identifies the upp locus as a mutational target that is capable of detecting a diverse spectrum of mutagenic events and further describes a tester strain, designated FU100 (his+, rfa, ΔuvrB, pkM101, 5-fluorouridine resistant) for use in the assay of the invention.