MS. JUAN LI, MD
Student, Health Care at Ave, Pittsburgh, PA
License number
Pennsylvania MT214549
Category
Student, Health Care
Type
Student in an Organized Health Care Education/Training Program
Address
Address
320 EAST Avenue NORTH, Pittsburgh, PA 15212
Phone
(412) 359-6907
Personal information
See more information about JUAN LI at radaris.comName
Address
Phone
801 Locust St, Philadelphia, PA 19107
1101 Spencer St, Philadelphia, PA 19141
600 Thomas Armor Dr, Windsor, PA 17366
Professional information
Overcoming Aav Vector Size Limitation Through Viral Dna Hetero-Dimerization
US Patent:
2002007, Jun 20, 2002
Filed:
Apr 20, 2001
Appl. No.:
09/838786
Inventors:
Liangwu Sun - Pittsburgh PA, US
Juan Li - Pittsburgh PA, US
Xiao Xiao - Wexfoxd PA, US
Juan Li - Pittsburgh PA, US
Xiao Xiao - Wexfoxd PA, US
International Classification:
C12P021/06
US Classification:
435/069100
Abstract:
A method for overcoming the packaging limitations of recombinant adeno-associated virus (AAV) particles through AAV heterodimer formation is provided. In the method, an expressed nucleic acid, typically a portion of a gene encoding a full-length therapeutic protein, or a functional derivative thereof, is split into two or more fragments by the insertion of one or more introns. Each intron is then split and each of the gene portions are inserted between AAV ITRs for packaging into recombinant adeno-associated virus particles. The recombinant viral particles are then co-infected into a target cell. Once inside the cell, the viral vectors form head-to-tail heterodimers through sequence homology of the inverted terminal repeats, thereby re-forming the intron. During mRNA maturation, the intron is spliced from the continuous DNA molecule, removing the intron and, thus, the intervening ITR sequences, thereby restoring the precise coding sequence of the expressed nucleic acid.