Inventors:
Richard Dauer - Longmont CO, US
Christina Evanson - Lafayette CO, US
Paul Friedrich - Apex NC, US
Hafez Hafezzadeh - Longmont CO, US
Ramakrishna Nalitham - Louisville CO, US
Stephen Schneider - Raleigh NC, US
Mark Schwindt - Boulder CO, US
Roger Snyder - Lafayette CO, US
Joseph White - Broomfield CO, US
Gregory Withers - Boulder CO, US
International Classification:
C07K 14/00
Abstract:
The formation of inactive, insoluble forms of peptide can be minimized or, alternatively, inactive, insoluble forms of peptide compounds, if present, can be converted into more physiologically active, soluble forms by dissolving peptide samples in aqueous base and then acidifying the aqueous mixture to precipitate the peptide in the presence of at least one of a salt and a co-solvent. Preferably, both a salt and co-solvent are present. By carrying out the precipitation relatively rapidly (at least in a first stage of acidifying in which the pH of the alkaline medium is reduced to a pH in the range of 6 to 7.5, after which acidification to a final desired pH, e.g., 3 to 6, can occur more slowly) at relatively low temperature, the dissolution characteristics of the resultant precipitated peptide are even further improved. The process is robust, consistent, and suitable for commercial scale manufacture of peptides.