Joseph Ryan White
Electrician at Everett St, Westminster, CO
License number
Colorado 102564
Issued Date
Jul 30, 1999
Renew Date
Mar 1, 2001
Expiration Date
Feb 28, 2003
Type
Journeyman Electrician
Address
Address
9193 Everett St, Westminster, CO 80021
Professional information
Methods And Compositions For Preparing Peptides With Excellent Solubility Characteristics In Aqueous Solution At Physiological Ph
US Patent:
2006016, Jul 27, 2006
Filed:
Dec 28, 2005
Appl. No.:
11/321035
Inventors:
Richard Dauer - Longmont CO, US
Christina Evanson - Lafayette CO, US
Paul Friedrich - Apex NC, US
Hafez Hafezzadeh - Longmont CO, US
Ramakrishna Nalitham - Louisville CO, US
Stephen Schneider - Raleigh NC, US
Mark Schwindt - Boulder CO, US
Roger Snyder - Lafayette CO, US
Joseph White - Broomfield CO, US
Gregory Withers - Boulder CO, US
Christina Evanson - Lafayette CO, US
Paul Friedrich - Apex NC, US
Hafez Hafezzadeh - Longmont CO, US
Ramakrishna Nalitham - Louisville CO, US
Stephen Schneider - Raleigh NC, US
Mark Schwindt - Boulder CO, US
Roger Snyder - Lafayette CO, US
Joseph White - Broomfield CO, US
Gregory Withers - Boulder CO, US
International Classification:
C07K 14/00
US Classification:
530300000, 530412000
Abstract:
The formation of inactive, insoluble forms of peptide can be minimized or, alternatively, inactive, insoluble forms of peptide compounds, if present, can be converted into more physiologically active, soluble forms by dissolving peptide samples in aqueous base and then acidifying the aqueous mixture to precipitate the peptide in the presence of at least one of a salt and a co-solvent. Preferably, both a salt and co-solvent are present. By carrying out the precipitation relatively rapidly (at least in a first stage of acidifying in which the pH of the alkaline medium is reduced to a pH in the range of 6 to 7.5, after which acidification to a final desired pH, e.g., 3 to 6, can occur more slowly) at relatively low temperature, the dissolution characteristics of the resultant precipitated peptide are even further improved. The process is robust, consistent, and suitable for commercial scale manufacture of peptides.