JOSEPH B CONNOLLY
Engineering in Dedham, MA
License number
Massachusetts 24749
Issued Date
Jul 28, 2015
Type
Engineer in Training
Address
Address
Dedham, MA 02026
Professional information
Non-Separation Specific Binding Chemiluminescent Assay
US Patent:
5783453, Jul 21, 1998
Filed:
Jun 29, 1995
Appl. No.:
8/496549
Inventors:
Eve H. Barlow - Holliston MA
Eddie Carroll - Waltham MA
Joseph E. Connolly - Dedham MA
Michael J. Lee - Sherborn MA
Richard A. Martinelli - Brighton MA
John T. Unger - Medfield MA
Eddie Carroll - Waltham MA
Joseph E. Connolly - Dedham MA
Michael J. Lee - Sherborn MA
Richard A. Martinelli - Brighton MA
John T. Unger - Medfield MA
Assignee:
Chiron Diagnostics Corporation - East Walpole MA
International Classification:
G01N 33543, G01N 33551, G01N 33553, C12G 100
US Classification:
436518
Abstract:
The assay described herein is predicated on an observation that when acridinium ester labeled tracers are bound to their corresponding binding conjugate immobilized on a metal oxide solid phase, the measurable chemiluminescent light emission of the labeled tracer bound to the solid phase is quenched as compared to the free fraction tracer that is unattached to the solid phase. According to the invention, a non-separation specific binding assay to detect or quantify the presence of an analyte is provided where the entire reaction mixture is flashed (including unreacted tracer) and modulated signal (because of the quench effect) is associated with a reference, thus determining the amount or presence of said analyte in said sample. Disadvantages inherent in heterogeneous assays employing multiple separations may be avoided using this non-separation method.
Mixed Luminescent Conjugate Test
US Patent:
5672475, Sep 30, 1997
Filed:
Jan 20, 1995
Appl. No.:
8/375466
Inventors:
Michael J. Lee - Sherborn MA
Howard H. Weetall - Rockville MD
Joseph E. Connolly - Dedham MA
Howard H. Weetall - Rockville MD
Joseph E. Connolly - Dedham MA
Assignee:
Chiron Diagnostics Corporation - E. Walpole MA
International Classification:
C12Q 168, G01N 3353
US Classification:
435 6
Abstract:
A method is provided for detecting or quantitating each of a plurality of substances or at least one substance and an internal reference material or control material in a test sample using at least two different luminescent labelled conjugates. Each luminescent labelled conjugate being characterized in being activated to emit light under different process conditions. The invention also provides for test kits containing at least two different luminescent labelled conjugates for detecting or quantitating presence or absence of at least two substances or at least one substance an internal reference material or control material in a test sample.