Inventors:
Edward Srour - Indianapolis IN
Esmail Zanjani - Reno NV
John E. Brandt - Indianapolis IN
Ronald Hoffman - Indianapolis IN
Assignee:
Indiana University Foundation - Bloomington IN
International Classification:
A61K 3514
Abstract:
Disclosed are human pluripotent hematopoietic stem cell (PHSC) enriched compositions and methods for obtaining and using the compositions. A substantially homogeneous population of human hematopoietic cells characterized as CD34. sup. +, HLA-DR. sup. -, and c-kit receptor positive (KR. sup. +) and capable of in vitro self-renewal and differentiation to members of at least the erythroid, myeloid, and megakaryocytic lineages, is obtained by separating the population from a cellular mixture, for example by cytometric cell sorting techniques. Also disclosed is a method of obtaining persistent maintenance of grafted human hematopoietic cells in a mammal, which includes grafting a mammal in utero with a cellular composition enriched in human hematopoietic cells characterized as CD34. sup. +, HLA-DR. sup. - and capable of in vitro self-renewal and differentiation to members of the lymphoid, myeloid, erythroid and megakaryocytic lineages.