Inventors:
Thomas Tuschl - Brooklyn NY, US
John Pena - New York NY, US
Cherin Sohn - Rego Park NY, US
Sara Hakim - New York NY, US
Janos Ludwig - Bonn-Venusberg, DE
Pavol Cekan - New York NY, US
Assignee:
The Rockefeller University - New York NY
International Classification:
C12Q 1/68, G01N 33/48
Abstract:
In one aspect, the invention relates to a method for fixing a short nucleic acid in a biological sample. In another aspect, the invention relates to a method for detecting a target short nucleic acid in a biological sample. The method includes contacting the biological sample with an aldehyde-containing fixative, and subsequently contacting the sample with a water-soluble carbodiimide. In a further aspect, the invention relates to a kit for fixing a short nucleic acid in a biological sample. The kit includes a support substrate for holding the sample; an aldehyde-containing fixative; and a water-soluble carbodiimide.