Inventors:
Paul Behrens - Ellicot City MD, US
John Thompson - Baltimore MD, US
Kirk Apt - Ellicot City MD, US
Joseph Pfeifer - Reisterstown MD, US
James Wynn - Columbia MD, US
James Lippmeier - Columbia MD, US
Jaouad Fichtali - Lexington KY, US
Jon Hansen - Lexington KY, US
Assignee:
MARTEK BIOSCIENCES CORPORATION - Columbia MD
International Classification:
C12P 7/64
Abstract:
Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.