JOHN MCINTOSH NINOS
Medical Practice in Tampa, FL
License number
Pennsylvania MD061502L
Category
Medicine
Type
Medical Physician and Surgeon
Address
Address
2
Tampa, FL 33618
Pennsylvania
Pennsylvania
Professional information
Inhibition Of Ship To Enhance Stem Cell Harvest And Transplantation
US Patent:
2006022, Oct 5, 2006
Filed:
May 28, 2004
Appl. No.:
10/709801
Inventors:
Caroline Desponts - Tampa FL, US
Joseph Wahle - Tampa FL, US
John Ninos - Tampa FL, US
William Kerr - Tampa FL, US
Joseph Wahle - Tampa FL, US
John Ninos - Tampa FL, US
William Kerr - Tampa FL, US
Assignee:
University of South Florida - Tampa FL
International Classification:
A61K 48/00
US Classification:
514012000, 514044000
Abstract:
The instant invention teaches the inhibition of SHIP expression, or function, for the increased efficacy of autologous stem cell transplants. In another embodiment, interference with SHIP function can be used to temporarily expand and mobilize the hematopoietic stem cell compartment to assist with leukapheresis, to promote hematopoietic recovery after myeloablation treatments, to deplete target stem cell clones (such a leukemic clones and other tumor stem cell types), and to deplete, or damage, the repopulating ability of the endogenous hematopoietic stem cell pool in order to allow transplanted hematopoietic stem cells to better home and engraft and to promote in vivo expansion and mobilization of other organ-specific stem cell populations (e.g., mesenchymal, mammary).
Novel Sh2Containing Inositol 5'-Phosphatase Isoform That Partners With The Grb2 Adapter Protein
US Patent:
2007022, Sep 27, 2007
Filed:
Mar 19, 2007
Appl. No.:
11/726028
Inventors:
William Kerr - Tampa FL, US
John Ninos - Tampa FL, US
John Ninos - Tampa FL, US
Assignee:
University of South Florida - Tampa FL
International Classification:
C12N 15/87, A61K 49/00, C12N 5/00, G01N 33/53, C07H 21/04
US Classification:
424009100, 435375000, 435377000, 435461000, 435007210, 536023100
Abstract:
SH2 containing inositol 5′-phosphate (SHIP) modulates the activation of immune cells after recruitment to the membrane by Shc and the cytoplasmic tails of receptors. A novel SHIP isoform of approximately 104 kd expressed in primitive stem cell populations (s-SHIP) is described. It was found that s-SHIP is expressed in totipotent embryonic stem cells to the exclusion of the 145-kd SHIP isoform expressed in differentiated hematopoietic cells. s-SHIP is also expressed in primitive hematopoietic stem cells, but not in lineage-committed hematopoietic cells. In embryoic stem cells, s-SHIP partners with the adapter protein Grb2 without tyrosine phosphorylation and is present constitutively at the cell membrane. It is postulated that s-SHIP modulates the activation threshold of primitive stem cell populations.