JOHN J ARRUDA
Plumbers in South Attleboro, MA
License number
Massachusetts 8214
Expiration Date
May 1, 1998
Type
Master Plumber
Address
Address
South Attleboro, MA 02703
Professional information
Hybridization-Ligation Assays For The Detection Of Specific Nucleic Acid Sequences
US Patent:
5800994, Sep 1, 1998
Filed:
Jul 24, 1996
Appl. No.:
8/685793
Inventors:
Richard A. Martinelli - Brighton MA
John C. Arruda - Attleboro MA
John C. Arruda - Attleboro MA
Assignee:
Chiron Diagnostics Corporation - East Walpole MA
International Classification:
C12Q 168, C12Q 170, C12P 1934, C07H 2104
US Classification:
435 6
Abstract:
A new method has been developed for conducting a gene probe assay. The preferred technique involves (1) using a gene amplification technique (e. g. , PCR) to multiply the gene sequence of interest and (2) using a hybridization--ligation detection methodology, wherein the sequences of probes hybridized to the target sequence allow for separation and detection (e. g. , probes might contain a combination of magnetic particles and acridinium esters) to determine if a specific sequence is present.