Inventors:
Richard A. Martinelli - Brighton MA
John C. Arruda - Attleboro MA
Assignee:
Chiron Diagnostics Corporation - East Walpole MA
International Classification:
C12Q 168, C12Q 170, C12P 1934, C07H 2104
Abstract:
A new method has been developed for conducting a gene probe assay. The preferred technique involves (1) using a gene amplification technique (e. g. , PCR) to multiply the gene sequence of interest and (2) using a hybridization--ligation detection methodology, wherein the sequences of probes hybridized to the target sequence allow for separation and detection (e. g. , probes might contain a combination of magnetic particles and acridinium esters) to determine if a specific sequence is present.