Inventors:
Karim Tabiti - Poecuing, DE
Gisela Betzl - Moosweg, DE
Richie Soong - Parap NJ, US
Randy P. Rasmussen - Salt Lake City UT, US
Deepika Marine Desilva - Salt Lake City UT, US
John G. Ward - Salt Lake City UT, US
Hallegh Page Millward - Riverton UT, US
Assignee:
Roche Diagnostics Corporation - Indianapolis IN
Idaho Technology, Inc. - Salt Lake City UT
International Classification:
C12Q 1/68, C12P 19/34
Abstract:
The invention provides a method for quantitative multiplex PCR of two targets whereby one target is present in at least 100-fold molar excess over that of the other target. The method further comprises the addition of a thermostable DNA polymerase with a final concentration of at least 0. 5 units/μl.