JOHN C RUTLEDGE, MD
Osteopathic Medicine at Y St, Sacramento, CA

2010024, Sep 23, 2010

May 31, 2006

11/916273

James W. Chan - Davis CA, US
Thomas R. Huser - Davis CA, US
Stephen M. Lane - Davis CA, US
John C. Rutledge - Davis CA, US
Douglas S. Taylor - Davis CA, US
Theodore Zwerdling - Davis CA, US
Ronald Li - Davis CA, US

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

G01J 3/44, G01N 33/48, G06F 17/18

702 19, 356 39, 356301, 702179

Raman spectra of cells, such as normal human T- and B-cells from peripheral blood or human tonsil and the corresponding transformed cells are obtained by optically trapping the cells and obtaining their Raman spectra. The trapped cells can be subjected to one, two, or more different excitation wavelengths, and each wavelength of the corresponding Raman spectra can be stored in a separate channel. In preferred embodiments, two spectra are subtracted from each other to give a difference spectrum and each channel is analyzed independently to characterize the trapped cell. Alternatively, the Raman spectrum can be subjected to Principal Component Analysis (PCA) in order to characterize the trapped cell. The trapped cell thus classified can be sorted, or further manipulated.