Inventors:
Peter S. Klein - Wynnewood PA, US
Jian Huang - Philadelphia PA, US
International Classification:
A61M 37/00, A61K 35/28, A61P 35/00, A61P 7/06, A61P 19/08, C12N 5/0775, A61P 35/02
US Classification:
604522, 435375, 435372, 435325, 424 937
Abstract:
The present invention provides a therapeutic approach to maintain and expand HSCs in vivo using currently available medications that target GSK-3 and mTOR. The present invention also provides a system and method for the ex vivo culturing of HSCs, where an mTOR inhibitor is combined with a GSK-3 inhibitor within the culturing conditions.