JIAN GUAN, M.D.
Osteopathic Medicine at Orange Ave, Orlando, FL

License number

Florida TRN#21399

Personal information

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Name

Address

Phone

Jian Guan

51 Old Landing Way APT D, Charlestown, MA 02129

Jian Guan

411 Newport Ave #2, Quincy, MA 02170

Jian Guan, age 62

5470 NW 186Th St, Miami Gardens, FL 33055

Jian Guan

32 Dwight St, Boston, MA 02118

(617) 306-4414

Jian Guan

290 Billings Rd #1004, Quincy, MA 02170

(617) 694-1738

Professional information

See more information about JIAN GUAN at trustoria.com

Jian Guan - Allston, MA

Work:

Brigham and Women's Hospital/Cardiovascular Division
Postdoctoral Research Fellow Boston University School of Medicine/Oncology Department - Boston, MA
Graduate Student Tongji Medical College/Surgery Department - Wuhan, CN
Graduate Student Tongji Hospital of Tongji Medical College - Wuhan, CN
Medical Student

Education:

Boston University
Doctor of Philosophy in Molecular Medicine Program Tongji Medical College
Doctor of Medicine in Oncology Tongji Medical College
Bachelor of Medicine

Skills:

Molecular Biology: DNA/RNA isolation, purification and amplification. PCR cloning with TA/Gateway technology. Co-immunoprecipitation and GST pull down assay. Quantitative PCR with SYBR Green technique. Construct design and cloning to achieve overexpression and knockdown (shRNA) of targeted genes in cell lines and animals via adenovirus and lentivirus. Western blotting, In situ Western blotting, ELISA, Flow cytometry, in vitro transcription, protein engineering, exosome isolation and characterization Cellular Biology: Culture of cancer cell lines (HepG2, Hep3B, MCF7, HEK cells). Establishment of stable transfected cell line. In vitro cancer assay: proliferation assay (Staining with proliferation markers: Ki67, Phospho-Histone 3; Flow cytometry with DAPI staining for cell cycle analysis; High-through put screening with CellTiter Glo luminescent assay); cell death assay (Caspase 3 immunoblotting, Flow cytometry assay with Annexin 5 staining, MTT assay); cell function assay: Mitochondrial function assay and lysotracker function. Cellular and Animal Imaging: Harvesting, fixing, trimming, processing, embedding, sectioning of animal and human samples. Immunohistochemistry staining. Immunofluorescence staining, Laser scanning confocal microscopy (running the confocal imaging core in CMRL), living cell imaging, organelle imaging; 2D echocardiography and color Doppler in cardiac imaging and function assessment. Whole animal imaging and non-invasive tracking of implanted cells. In Vivo: Collection of serum/plasma/whole blood, tissues, primary tumor material and metastases from animal models. Mouse anesthesia and surgery. Tumor xenotransplantation in immunosuppressed nude mice, Pharmacological agent administration to mice or rats via IV, IP, SC, IM and PO. Establishment of plasma cell tumor transgenic mouse model. Software: GraphPad Prism, SPSS.