MS. JI Y. LEE, PHARMACIST
Pharmacy at Goshen Rd, Gaithersburg, MD
License number
Maryland 14248
Category
Pharmacy
Type
Pharmacist
Address
Address
20211 Goshen Rd, Gaithersburg, MD 20879
Phone
(301) 670-1631
(301) 670-1642 (Fax)
(301) 670-1642 (Fax)
Professional information
Chemiluminescent Enzyme Assay Method And Apparatus
US Patent:
8492101, Jul 23, 2013
Filed:
Apr 16, 2010
Appl. No.:
12/761721
Inventors:
Ji Hoon Lee - Gaithersburg MD, US
Assignee:
Luminescent MD, LLC - Gaithersburg MD
International Classification:
G01N 33/53, G01N 33/533
US Classification:
435 71, 435 792
Abstract:
A chemiluminescent enzyme immunoassay method for quantifying antigen or antibody using 1,1′-oxalyldiimidazole (ODI) derivative or 1,1′-oxalyldisodium benzoate (ODB) derivative chemiluminescence (CL) detection was developed. Also, various enzymes were quantified using ODI derivative or DOB derivative CL detection. Fluorescent compound formed from a substrate (non-fluorescent compound) through the enzyme assay methods emitted CL when the fluorescent compound received energy from high-energy intermediate formed in ODI derivative or ODB derivative CL reaction.
Method For Enhancing Enzyme Assays
US Patent:
2012023, Sep 20, 2012
Filed:
Mar 16, 2012
Appl. No.:
13/422650
Inventors:
Ji Hoon LEE - Gaithersburg MD, US
International Classification:
G01N 33/566, G01N 33/53
US Classification:
435 792, 435 71
Abstract:
Additives capable of controlling the reaction rate between substrate and enzyme to form a dye in enzyme assays with absorbance, chemiluminescence, and fluorescence are effective tools for optimizing enzyme assays. A dye can be formed rapidly from the reaction between enzyme and substrate in the presence of a catalyst. Using relatively high concentration of dye formed from the rapid reaction, trace levels of analytical materials can be quantified using absorbance, chemiluminecence and fluorescence detection. A dye can be formed from a relatively slow reaction between enzyme and substrate in the presence of a surfactant such as Triton X-100 and β-cyclodextrin. Using relatively low concentration of dye from the slow reaction, a high concentration of analytical material can be quantified without any dilution using absorbance, chemiluminescence, and fluorescence.