Inventors:
Lawrence J. Wangh - Auburndale MA, US
John Rice - Quincy MA, US
J. Aquilles Sanchez - Framingham MA, US
Kenneth Pierce - Natick MA, US
Jesse Salk - Seattle WA, US
Arthur Reis - Arlington MA, US
Cristina Hartshorn - Needham MA, US
Assignee:
BRANDEIS UNIVERSITY - Waltham MA
International Classification:
C12Q 1/68
Abstract:
Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.