Inventors:
Saulius Butenas - Burlington VT
Jeffrey H. Lawson - Durham NC
Kenneth G. Mann - Shelburne VT
Assignee:
Haematologic Technologies, Inc. - Essex Jct. VT
International Classification:
C12Q 156, C12Q 100, C07D27304, C07D25708
Abstract:
The present invention encompasses compounds of formula I: ##STR1## and the pharmaceutically acceptable non-toxic salts thereof; wherein R. sub. 1 is hydrogen, lower alkyl, alkenyl, alkynyl, cycloalkyl, alkylcycloalkyl, cycloalkylalkyl, or phenylalkyl;
R. sub. 2 is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, alkylcycloalkyl, cycloalkylalkyl, or phenylalkyl; or
NR. sub. 1 R. sub. 2 forms a nitrogen heterocycle; and
R. sub. 3 is hydrogen, an amino acid or a peptide residue.
These compounds may be employed as substrates in assays for determining proteolytic enzyme activity or as enzyme inhibitors. The invention also encompasses methods for determining proteolytic enzyme activity using the compounds of formula I.