DR. JEFFREY GIMBLE, MD, PHD
Osteopathic Medicine at Canal St, New Orleans, LA

2014001, Jan 16, 2014

Dec 13, 2012

13/713784

Jeffrey M. Gimble - New Orleans LA, US
Forum S. Shah - Baton Rouge LA, US
Xiying Wu - New Orleas LA, US

Board of Supervisors of Louisiana State University and Agricultural and Mechanical College - Baton Rouge LA

C12N 5/0775

435366, 435378, 435325, 435377

A simple method was developed to extract adipose-derived stromal stem cells (ASCs) without using enzymatic digestion of the collagen in adipose tissue. The resulting ASCs isolated by the simple wash method have lower levels of CD34 expression, a hematopoietic stem cell marker, as compared to ASCs isolated using conventional enzymatic digestion using collagenase. This characteristic is consistent with non-enzymatically treated stem cells obtained from bone marrow aspirates, which are negative for CD34. Additionally, the washed ASCs have higher levels of CD44 expression, a hyaluronate receptor, and lower levels of contaminating hematopoietic cells, as evidenced by low CD45 expression, as compared to enzymatically digested cells. The cells produced by this simple method can be used therapeutically for allogenic or autologous tissue regeneration, and can be administered using any pharmaceutically acceptable carrier. In addition, the cells can be administered in a matrix, lattice, scaffold, or other biologically compatible materials.