Inventors:
Lonnie D. Shea - Evanston IL, US
Jeffrey Bonadio - San Diego CA, US
David J. Mooney - Ann Arbor MI, US
Martin C. Peters - Ann Arbor MI, US
Assignee:
The Regents of the University of Michigan - Ann Arbor MI
International Classification:
A61N 43/04, A61K 63/00, A61K 9/14, A61K 45/00, A61F 13/00, C12P 21/06, C12N 15/00, C12N 5/00, A61K 38/00
US Classification:
514 44, 424 931, 424422, 424484, 424 851, 435 691, 4353201, 435325, 435455, 514 2, 514 54
Abstract:
Disclosed are particular 3-dimensional structural matrices containing nucleic acids, various fabrication processes and methods for the prolonged release of nucleic acids in various biological environments. The nucleic acid-matrix materials are created such that they maintain a defined space, allowing cellular migration, transfection and proliferation to occur in a controlled manner. The fabrication processes provide for both high incorporation efficiencies and control over the sustained nucleic acid release. The resultant nucleic acid-containing structural matrices are thus particularly useful in in vivo cell transfection and gene expression in the context of gene therapy.