Inventors:
Saverio Carl Falco - Arden DE, US
Omolayo O. Famodu - Newark DE, US
Jan Antoni Rafalski - Wilmington DE, US
Michael Lee Ramaker - Greenville DE, US
Mitchell Christian Tarczynski - West Des Moines IA, US
Catherine Thorpe - Cambridgeshire, GB
Assignee:
E. I. du Pont de Nemours and Company - Wilmington DE
International Classification:
A01H 1/00, A01H 4/00, C12N 15/82, C12N 5/10, C07H 21/04
US Classification:
800278, 4353201, 435468, 435410, 435419, 435 691, 435183, 536 231, 536 232, 536 234, 536 236, 536 241, 800281, 800290, 800295, 800298
Abstract:
This invention relates to a nucleic acid fragment encoding a plant 5-methyltetra-hydropteroyltriglutamate-homocysteine methyltransferase or methionine synthase. The invention also includes chimeric genes, a first encoding a plant methionine synthase (MS) gene, a second encoding a plant cystathionine γ-synthase (CS) gene, a third encoding feedback-insensitive aspartokinase (AK) or bifunctional feedback-insensitive aspartokinase-homoserine dehydrogenase (AK-HDH), which is operably linked to a plant chloroplast transit sequence, and a fourth encoding a methionine-rich protein, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of methionine in the seeds of transformed plants are provided.