Inventors:
JAMES WEST - NASHVILLE TN, US
Assignee:
MICE WITH HORNS, LLC - DELRAY BEACH FL
International Classification:
C12N 15/85
US Classification:
800 15, 435325, 800 13, 800 14, 800 16, 800 17, 800 18, 800 19, 800 20, 800 21
Abstract:
Disclosed are materials and methods for creating customizable traits in animals. In the demonstration of the principle of the subject invention, a keratin-14 specific promoter is used with, red fluorescent protein in the loxp cassette, dominant black (ΔG23) beta defensin 103 in the pigment cassette, and an SV40 (with intron) polyadenylation sequence. When Cre recombinase (or HTNCre) is applied to the animal's skin in a carrier base (e.g., lipid bilayers), fur is permanently genetically modified to turn black in the shape in which it was applied.