Inventors:
Jason A. Papin - Charlottesville VA, US
James W. Mandell - Charlottesville VA, US
George F. Glass - Charlottesville VA, US
Assignee:
UNIVERSITY OF VIRGINIA PATENT FOUNDATION - Charlottesville VA
International Classification:
G01N 33/53, G01N 21/64, C12Q 1/02
Abstract:
The present invention provides a novel method called Sequential IMmunoPeroxidase Labeling and Erasing (SIMPLE) that enables the simultaneous visualization of at least five markers within a single tissue section. Utilizing the alcohol-soluble peroxidase substrate 3-amino-9-ethylcarbazole (AEC), combined with a rapid non-destructive method for antibody-antigen dissociation, the present application discloses the ability to erase the results of a single immunohistochemical stain while preserving tissue antigenicity for repeated rounds of labeling. The present invention also provides methods for visualizing multiple antigens simultaneously.