Inventors:
James Versalovic - Houston TX, US
Carissa Michelle Thomas - Houston TX, US
Eamonn Connolly - Lindingo, SE
International Classification:
A61K 35/74, A61P 1/00, C12N 1/20, A61P 15/02, G01N 33/53, C12Q 1/04, A61P 29/00, A61P 1/04
US Classification:
424 9345, 435 792, 435 34, 4352529
Abstract:
A method is provided of selecting specific probiotic lactic acid bacteria producing histamine and the use of such strains for beneficial effects for mammals. The method includes selecting a lactic acid bacterial strain for use in the local production of histamine in a mammal, and further comprises screening bacteria for the presence of an active histidine operon and selecting a strain which has an active histidine operon and is capable of producing histamine. Preferably said strain is selected for its ability to produce histamine at a level of greater than 250 pg/ml. The present invention further provides products comprising the strains obtainable by the selection methods of the invention for use in the local production of histamine in a mammal, in particular for use in the treatment or prophylaxis of inflammatory conditions.