JAMES LEE, NP-C
Nursing in Knoxville, TN

7932437, Apr 26, 2011

May 15, 2007

11/748531

James Weifu Lee - Knoxville TN, US

C12N 15/82, C12N 1/12, C12N 1/20

800296, 4352572

A designer proton-channel transgenic alga for photobiological hydrogen production that is specifically designed for production of molecular hydrogen (H) through photosynthetic water splitting. The designer transgenic alga includes proton-conductive channels that are expressed to produce such uncoupler proteins in an amount sufficient to increase the algal Hproductivity. In one embodiment the designer proton-channel transgene is a nucleic acid construct () including a PCR forward primer (), an externally inducible promoter (), a transit targeting sequence (), a designer proton-channel encoding sequence (), a transcription and translation terminator (), and a PCR reverse primer (). In various embodiments, the designer proton-channel transgenic algae are used with a gas-separation system () and a gas-products-separation and utilization system () for photobiological Hproduction.

7642405, Jan 5, 2010

May 15, 2007

11/748550

James Weifu Lee - Knoxville TN, US

C12N 15/63, C12N 15/82

800296, 800285, 800286, 800287

A switchable photosystem-II designer algae for photobiological hydrogen production. The designer transgenic algae includes at least two transgenes for enhanced photobiological Hproduction wherein a first transgene serves as a genetic switch that can controls photosystem II (PSII) oxygen evolution and a second transgene encodes for creation of free proton channels in the algal photosynthetic membrane. In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (), a inducible promoter (), a PSII-iRNA sequence (), a terminator (), and a PCR reverse primer (). In other embodiments, the PSII-iRNA sequence () is replaced with a CF-iRNA sequence (), a streptomycin-production gene (), a targeting sequence () followed by a proton-channel producing gene (), or a PSII-producing gene (). In one embodiment, a photo-bioreactor and gas-product separation and utilization system produce photobiological Hfrom the switchable PSII designer alga.

7973214, Jul 5, 2011

Sep 25, 2007

11/903919

James Weifu Lee - Knoxville TN, US

UT-Battelle, LLC - Oak Ridge TN

A01H 5/00, C12N 15/52, C12N 15/82

800284, 800288, 800295, 800296, 800298, 4352572, 435419, 435468

The present invention provides a revolutionary photosynthetic ethanol production technology based on designer transgenic plants, algae, or plant cells. The designer plants, designer algae, and designer plant cells are created such that the endogenous photosynthesis regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic water splitting and proton gradient-coupled electron transport process are used for immediate synthesis of ethanol (CHCHOH) directly from carbon dioxide (CO) and water (HO). The ethanol production methods of the present invention completely eliminate the problem of recalcitrant lignocellulosics by bypassing the bottleneck problem of the biomass technology. The photosynthetic ethanol-production technology of the present invention is expected to have a much higher solar-to-ethanol energy-conversion efficiency than the current technology and could also help protect the Earth's environment from the dangerous accumulation of COin the atmosphere.

6905586, Jun 14, 2005

Jan 28, 2002

10/055881

James W. Lee - Knoxville TN, US
Thomas G. Thundat - Knoxville TN, US

UT-Battelle, LLC - Oak Ridge TN

C12Q001/68, G01N027/26

204600, 204450, 204452, 204453, 204603, 204604, 204643, 204547, 435 6, 4352872

An apparatus and method for performing nucleic acid (DNA and/or RNA) sequencing on a single molecule. The genetic sequence information is obtained by probing through a DNA or RNA molecule base by base at nanometer scale as though looking through a strip of movie film. This DNA sequencing nanotechnology has the theoretical capability of performing DNA sequencing at a maximal rate of about 1,000,000 bases per second. This enhanced performance is made possible by a series of innovations including: novel applications of a fine-tuned nanometer gap for passage of a single DNA or RNA molecule; thin layer microfluidics for sample loading and delivery; and programmable electric fields for precise control of DNA or RNA movement. Detection methods include nanoelectrode-gated tunneling current measurements, dielectric molecular characterization, and atomic force microscopy/electrostatic force microscopy (AFM/EFM) probing for nanoscale reading of the nucleic acid sequences.

2004011, Jun 17, 2004

Oct 22, 2003

10/690838

Danny Day - Atlanta GA, US
James Lee - Knoxville TN, US

C01B031/02, C10B057/04, C10B051/00, C10J001/00

048/1970FM, 423/44500R, 201/025000, 585/241000, 048/19700R

This invention relates a series of steps to provide an economical production of a carbon based fertilizer and soil amendment made during the capture of greenhouse gases from the combustion of fossil and non fossil fuels. The invention uses biomass and other carbonaceous sources through pyrolytic conversion to gases and charcoal, to allow for the further production of co-products, such as hydrogen and ammonia. The invention also relates to the combination of hydrated ammonia, combustion flue gas exhaust, and charcoal, provide for the conversion of the charcoal into a valued added soil amendment to return essential trace minerals and plant nutrients to the soil. The ability to produce a large volume carbon co-product while removing mandated emissions and producing renewable based hydrogen provides an economic gain to a large number small and large businesses and increase the chance of achieving significant reductions in greenhouse gas emissions.