Inventors:
Marshall A. Lichtman - Rochester NY
James K. Brennan - Rochester NY
International Classification:
C12B 300, C12K 900
Abstract:
Human cell lines have been obtained which elaborate colony stimulating activity (CSA) for marrow cells, i. e. , which enable cells to be grown in vitro for use in bioassay methods in diagnostics and therapy. One cell line, GCT, was obtained from a lung metastasis of a fibrous histiocytoma and another, RC4 from a monocyte enriched fraction of normal blood. These cell lines provide a continuous source of large quantities of conditioned medium from purification of CSA and is more potent in stimulating human narrow growth in vitro than conditioned mediums heretofore available, i. e. , human embryo kidney cell or human leukocyte feeder layers, having finite life spans in culture.