Inventors:
David A. Zarling - Menlo Park CA
Michel J. Rossi - Lausanne, CH
Norman A. Peppers - Belmont CA
James Kane - Lawrenceville NJ
Gregory W. Faris - Menlo Park CA
Mark J. Dyer - San Jose CA
Steve Y. Ng - San Francisco CA
Luke V. Schneider - Half Moon Bay CA
Assignee:
SRI International - Menlo Park CA
International Classification:
G01N 3353, G01N 33537, G01N 33543
Abstract:
The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical and environmental sampling techniques.