DR. JAMES DAVIS CONNOR, MD
Medical Practice at Avenida Cresta, La Jolla, CA
License number
California C32219
Category
Medical Practice
Type
Pediatrics
Address
Address
6423 Avenida Cresta, La Jolla, CA 92037
Phone
(858) 922-5968
Professional information
Method Of Identifying Viruses
US Patent:
5075214, Dec 24, 1991
Filed:
Nov 8, 1988
Appl. No.:
7/268761
Inventors:
James D. Connor - La Jolla CA
Pramila Walpita - San Diego CA
Pramila Walpita - San Diego CA
Assignee:
Regents of the University of California - Berkeley CA
International Classification:
C12Q 170
US Classification:
435 5
Abstract:
A method is described for identifying a virus infecting a host cell material, where the virus does not induce adequate or consistent host cell protein synthesis suppression, which comprises contacting a sample of the virus infected host cell material with an effective host cell protein synthesis suppression agent, allowing the virus to produce proteins characteristic of that virus in the host cell material following suppression of the host cell protein synthesis, analyzing the viral proteins so produced, obtaining from such analysis a record containing indicia uniquely characteristic of those viral proteins, and identifying the virus which produced the viral proteins by comparing the unique indicia with a library of predetermined like indicia, each of which has previously been identified with a specific virus. Preferably the host cell protein synthesis suppression agent is sodium chloride. Analysis of the viral proteins is preferably accomplished radiolabeling the proteins and separating them by electrophoresis, most preferably SDS-PAGE.