Medical School Medical College of Pennsylvania Graduated: 1986 Hospital Of The University Of Pennsylvania Meml Sloan-Kettering Cancer Ctr Ny Hosp-Cornell Med Ctr
James Luketich, Pittsburgh PA
Work:
UPMC Presbyterian, Digestive Disorders Center 200 Lothrop St, Pittsburgh, PA 15213Hillman Cancer Center 5115 Centre Ave, Pittsburgh, PA 15232Univ. of Pittsburgh Physicians 5200 Centre Ave, Pittsburgh, PA 15232
James D Luketich, Pittsburgh PA
Specialties:
Surgeon
Address:
200 Lothrop St, Pittsburgh, PA 15213
Education:
Medical College of Pennsylvania - Doctor of Medicine New York Presbyterian Hospital/Weill Cornell Medical Center - Residency - Cardiothoracic Surgery Memorial Sloan-Kettering Cancer Center - Residency - Cardiothoracic Surgery Hospital of the University of Pennsylvania - Internship - Surgery
Board certifications:
American Board of Surgery Certification in Surgery, American Board of Thoracic Surgery Certification in Thoracic and Cardiac Surgery (Thoracic Surgery)
Pcr Method And Related Apparatus
US Patent:
2006020, Sep 14, 2006
Filed:
May 12, 2006
Appl. No.:
11/433722
Inventors:
Tony Godfrey - Pittsburgh PA, US James Luketich - Pittsburgh PA, US Siva Raja - Pittsburgh PA, US Lori Kelly - Jeanette PA, US Sydney Finkelstein - Pittsburgh PA, US
International Classification:
C12Q 1/68, C12P 19/34
US Classification:
435006000, 435091200
Abstract:
A method for balancing multiplexed PCR methods is provided. In the method, two or more sequential temporal PCR stages are used to effectively separate two or more PCR reactions in a single tube as an alternative to primer limiting to modulate the relative rate of production of a first amplicon by a first primer set and a second amplicon by a second primer set during the first and second amplification stages. Also provided are rapid RT-PCR methods that find particular use in intraoperative diagnoses and prognoses, for instance in diagnosing malignant esophageal adenocarcenoma by determining expression levels of carcinoembryonic antigen (CEA) in sentinel lymph nodes.
Pcr Method
US Patent:
2003001, Jan 23, 2003
Filed:
Mar 4, 2002
Appl. No.:
10/090326
Inventors:
Tony Godfrey - Pittsburgh PA, US James Luketich - Pittsburgh PA, US Siva Raja - Pittsburgh PA, US Lori Kelly - Jeanette PA, US Sydney Finkelstein - Pittsburgh PA, US
International Classification:
C12Q001/68, C12P019/34
US Classification:
435/006000, 435/091200
Abstract:
A method for balancing multiplexed PCR methods is provided. In the method, two or more sequential temporal PCR stages are used to effectively separate two or more PCR reactions in a single tube as an alternative to primer limiting to modulate the relative rate of production of a first amplicon by a first primer set and a second amplicon by a second primer set during the first and second amplification stages. Also provided are rapid RT-PCR methods that find particular use in intraoperative diagnoses and prognoses, for instance in diagnosing malignant esophageal adenocarcenoma by determining expression levels of carcinoembryonic antigen (CEA) in sentinel lymph nodes.