Inventors:
PRECISION BIOSCIENCES, INC. - , US
Michael G. NICHOLSON - Chapel Hill NC, US
James Jefferson SMITH - Durham NC, US
Assignee:
PRECISION BIOSCIENCES, INC. - Durham NC
International Classification:
C12N 9/16
US Classification:
435462, 435196, 536 232, 435468
Abstract:
Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.