Inventors:
Russell N. Van Gelder - Des Peres MO
Mark E. Von Zastrow - San Carlos CA
Jack D. Barchas - New York NY
James D. Eberwine - New York NY
Assignee:
Board of Trustees of Leland Stanford University - Stanford CA
International Classification:
C12Q 168, C12N 163, C07H 2104
Abstract:
This invention relates to the use of promoters for ribonucleic acid amplification and other genetic manipulations. Processes are provided wherein complementary deoxyribonucleic acid (cDNA) is synthesized from a ribonucleic acid (RNA) sequence using a complementary primer linked to an RNA polymerase promoter region complement and then anti-sense RNA (aRNA) is transcribed from the cDNA by introducing an RNA polymerase capable of binding to the promoter region. Additional processes using the resulting aRNA are also described.