J. Fred Kolhouse
Physician at 19 Ave, Denver, CO

5800979, Sep 1, 1998

Mar 20, 1996

8/619091

J. Fred Kolhouse - Denver CO
John C. Deutsch - Denver CO

C12Q 100, C12Q 132

435 4

A method for determination of in vivo concentrations in a body fluid of one or more folate co-enzymes is provided including the steps of (a) combining a known amount of at least one folate internal standard with a body fluid collected in vitro, said body fluid containing at least one co-enzyme; (b) at least partially purifying the endogenous and internal standard folates from other components in the in vitro body fluid; (c) quantitating the endogenous folate coenzymes in the purified in vitro body fluid of step (b) by gas chromatography/mass spectrometry analysis; and (d) determining the in vivo concentration of folate coenzymes by correcting the quantitated in vitro endogenous concentrations for endogenous losses as reflected by the losses in the known amounts of the internal standards. Quantitation of total folates or single folate co-enzymes aids in the diagnosis and treatment of a number of clinical conditions involving folate deficiencies including megaloblastic anemia, neurological and psychiatric disorders and vitamin B. sub. 12 deficiency.

5559038, Sep 24, 1996

May 4, 1994

8/238754

J. Fred Kolhouse - Denver CO
John C. Deutsch - Denver CO

The Regents of the University of Colorado - Boulder CO

G01N 3000

436 86

Method for quantifying the amounts of oxidized sulfhydryl amino acids, particularly cysteine sulfinic acid, cysteic acid, homocysteine sulfinic acid and homocysteic acid, for example, in biological samples are provided. The oxidized sulfhydryl amino acids are measured by gas chromatography/mass spectrometry after derivatization, preferably using stable isotope internal standards. Deviation from normal individuals' levels of oxidized sulfhydryl amino acids in serum are indicative of certain neuropsychiatric disorders, the biochemical origin of which can be diagnosed using methods disclosed herein, particularly when relative levels of these and/or other sulfur amino acids are measured.

5234905, Aug 10, 1993

Feb 22, 1991

7/669849

J. Fred Kolhouse - Denver CO
John C. Deutsch - Denver CO

University of Colorado Foundation, Inc. - Boulder CO

A61K 3702, C07K 1514

514 8

A method for extending soluble CD4 serum half-life in mammals is described. The method comprises modifying soluble CD4 glycosylation so as to inhibit clearance from serum. In a preferred embodiment, clearance by hepatocyte galactose receptors is inhibited by removal of soluble CD4 terminal sialic residues followed by oxidation of exposed galactose residues. The modified soluble CD4 molecules are demonstrated to possess extended serum half-life.

5212096, May 18, 1993

Feb 19, 1992

7/838356

J. Fred Kolhouse - Denver CO
John C. Deutsch - Denver CO

University of Colorado Foundation, Inc. - Boulder CO

G01N 3300

436 93

A method for quantifying in vivo ascorbate concentration in body fluids uses gas chromatography/mass spectrometry (GC/MS). Further provided is a method for measuring redox potentials of body fluid by determining the ratio of in vivo concentration of ascorbate to one or more of its metabolites.

5506147, Apr 9, 1996

Nov 28, 1994

8/345534

J. Fred Kolhouse - Denver CO
John C. Deutsch - Denver CO
Vincent Guay - Montreal, Quebec, CA
James P. Ounsworth - Pierrefonds, Quebec, CA

G01N 33483

436 86

A bentiromide test for evaluation of pancreatic function is provided which includes the steps of administering a stable, non-radioactive isotope of PABA along with the bentiromide and measuring the natural PABA and PABA isotope by gas chromatography mass spectroscopy (GC/MS). In a preferred embodiment, xylose is also administered and measured in the sample by GC/MS.