Inventors:
Inder J. Chopra - Los Angeles CA
Assignee:
Research and Education Institute, Inc. - Torrance CA
International Classification:
G01N 3356, G01N 3356, G01N 3360, G01T 100
Abstract:
This invention relates to a highly accurate, rapid and simple estimation of thyroxine (T. sub. 4) directly from blood serum and also relates to the accurate measurement of triiodo-L-thyronine (T. sub. 3) directly from blood serum. More specifically, the invention relates to a rapid, specific and reliable radioimmunoassay (RIA) technique for measurement of both T. sub. 4 and T. sub. 3 in unextracted serum. The method requires very small amounts of serum, e. g. , 25 microliters (. mu. l) to measure T. sub. 4 concentration in nearly all specimens representing clinical states of eu-, hypo- and hyperthyroidism, and 250. mu. l to measure T. sub. 3 concentrations in specimens representing most clinical states.