HOWARD N STEINBERG
Broker in Brookline, MA
License number
Massachusetts 9030430
Issued Date
May 12, 1999
Expiration Date
Jun 28, 2003
Type
Salesperson
Address
Address
Brookline, MA 02445
Professional information
Assay To Detect The Presence Of Live Virus In Vitro
US Patent:
5073481, Dec 17, 1991
Filed:
Nov 30, 1989
Appl. No.:
7/443823
Inventors:
Jerome B. Zeldis - Newton Highlands MA
Robert P. Gale - Bel Air CA
Howard N. Steinberg - Brookline MA
Robert P. Gale - Bel Air CA
Howard N. Steinberg - Brookline MA
Assignee:
Beth Israel Hospital Association - Boston MA
International Classification:
C12Q 170, C12Q 102, C12N 704, A61K 3912
US Classification:
435 5
Abstract:
An assay to detect the presence of live hepatitis viruses in vitro. Bone marrow cells of leukemic cell line cells are exposed to a body fluid or biological preparation to be tested and the cells are placed in suspension. When using bone marrow cells, growth factors to the bone marrow stem cells are added. It has been determined that presence of a live hepatitis virus suppresses the growth of colonies of the stem cells. Therefore, if the number of colonies growing in the mixture are less than that number present in a culture of cells exposed to a sample that has been determined to contain no live virus, live hepatitis virus is present in the sample tested. The assay is particularly useful to determine the presence of live hepatitis B virus in a vaccine.
Assay To Detect The Presence Of Live Non-A, Non-B Hepatitis Agents In Vitro
US Patent:
4952494, Aug 28, 1990
Filed:
Dec 6, 1988
Appl. No.:
7/280490
Inventors:
Jerome B. Zeldis - Carmichael CA
Robert P. Gale - Bel Air CA
Howard N. Steinberg - Brookline MA
Robert P. Gale - Bel Air CA
Howard N. Steinberg - Brookline MA
Assignee:
Beth Isreal Hospital Association - Boston MA
International Classification:
C12Q 170, C12Q 102, C12Q 118
US Classification:
435 5
Abstract:
An assay to detect the presence of live non-A, non-B hepatitis virus in vitro. Bone marrow cells are exposed to a body fluid or biological preparation to be tested and the cells are placed in suspension. When using bone marrow cells, growth factors are added to the bone marrow stem cells. Therefore, if the number of colonies growing in the mixture are less than that number present in the culture of cells exposed to a sample that has been determined to contain no live virus, live non-A, non-B hepatitis virus is present in the sample tested. The assay is particularly useful to determine the presence of live non-A, non-B, hepatitis virus in a vaccine.