Inventors:
Paul R. August - Danville NH, US
Pamela J. Keagle - Leominster MA, US
Henry Long - Cambridge MA, US
Anna Wiencis Montmayeur - Waltham MA, US
Katherine Call - Malden MA, US
Michael Draper - Plaitsow NH, US
Assignee:
Aventis Pharmaceuticals Inc. - Bridgewater NJ
International Classification:
C12P 19/34, C12Q 1/68, C07H 21/02, C07H 21/04, C07H 21/00
US Classification:
435 912, 435 6, 435 911, 536 231, 536 243, 536 2433, 536 253, 536 2532
Abstract:
The present invention relates to an automated method of transposon-mediated multiplex sequencing of DNA fragments inserted into a vector. It relates more particularity to an increased efficiency in such automated methods, where the increased efficiency is obtained by screening out before the sequencing those constructs in which the transposon inserted into the vector sequence. This prevents a waste of time and resources in performing reactions sequencing the vector instead of the DNA fragments of interest.