DR. HARVEY I. CANTOR, M.D.
Medical Practice at Binney St, Boston, MA

4559310, Dec 17, 1985

May 20, 1983

6/496543

Harvey I. Cantor - Boston MA
Gary Nabel - Cambridge MA

Dana Farber Cancer Institute - Boston MA

G01N 3354, C12Q 129

436519

The present invention is directed to an in vitro assay, useful in determining the effectiveness of anti-allergy compounds and/or useful in measuring the degree of sensitivity of a patient to particular allergens. The present invention permits potential anti-allergy agents to be assayed in a number of ways. For example, the binding and dissociation rates of IgE to the mast cells in the presence and the absence of the substance being tested may be measured thereby giving a direct indication of that substance's ability to interfere with the IgE binding reaction. Another measure of a substance's potential as an anti-allergy agent is based upon the release of mediators or other compounds from the mast cells after sensitization by the allergen and exposure of the sensitized cells to the allergen. The present invention generally involves the following steps: (a) sensitizing cloned mast cells to an allergen; (b) exposing sensitized mast cells to the allergen in the presence of a test anti-allergy agent; and (c) measuring the reaction products of step (b) for an indication of test compound effect.

4613459, Sep 23, 1986

Oct 20, 1982

6/435510

Harvey I. Cantor - Boston MA
Gary Nabel - Cambridge MA

Dana-Farber Cancer Institute - Boston MA

C07K 328, C12N 500, C12N 502, C12N 138

530351

A family of growth factors, methods of making and using such growth factors in in vivo treatment of living mammalian cells, and products incorporating such growth factors, are disclosed. The growth factors were isolated from the supernatant fluid obtained by incubation of inducer T-cells in the presence of a suitable antigen or mitogen. The growth factors disclosed include a 14 Kd polypeptide which stimulates growth of both T-cells and B-cells, a 50 Kd polypeptide which stimulates B-cells to multiply and secrete immunoglobulins, and a 45 Kd growth factor which stimulates secretion of immunoglobulins by B-cells and stimulates rapid growth of certain mast cells, stem cells and certain other types of cells.